Fig. 3

CircDOCK1 serves as a miR-339-3p sponge. A Linear DOCK1 expression in OS tissues. B Association between circDOCK1 and DOCK1 (P = 0.207). C Suppression and overexpression of circDOCK1 had no modulatory effect on linear DOCK1 RNA expression in OS cells (normalized to si-NC or vector). D Suppression and overexpression of circDOCK1 had no modulatory effect on linear DOCK1 protein expression in OS cells. E Prediction of potential target miRNAs possibly binding to circDOCK1 with CircInteractome and starBase. F After pulldown with a circDOCK1-specific probe, miRNA expression was measured in lysates of MG63 and U2OS cells (normalized to the oligo probe). G PITA and TargetScan predictions of the target genes of miR-339-3p. H Combination of the prediction results with the GSE36001 RNA microarray analysis results. I Expression of potential targets of miR-339-3p in MG63 and U2OS cells post transfection with the miR-339-3p mimic or circDOCK1 siRNA (normalized to si-NC or miR-NC). J DOCK1 RNA expression was measured by qRT–PCR post transfection of the siRNA (normalized to si-NC). K RNA expression of IGF1R in MG63 and U2OS cells post transfection with the DOCK1 siRNA (normalized to si-NC). The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns: nonsignificant