Fig. 5

CircDOCK1 regulates malignant transformation via the miR-339-3p/IGF1R axis in vitro. A Hypothetical and mutant binding sites in miR-339-3p for IGF1R (lower) and circDOCK1 (upper). B Luciferase reporter assay detecting the binding of miR-339-3p to circDOCK1 and IGF1R in MG63 cell lines (normalized to miR-NC). C RIP with an anti-AGO2 antibody in the MG63 cell line evaluating the transcript levels of circDOCK1 and miR-339-3p (left). Western blot analysis evaluating the AGO2 protein level (right) (normalized to a control). D-E The mRNA (D) and protein (E) levels of IGF1R in MG63 cell lines post transfection (normalized to IgG). F CCK-8 assay. G EdU incorporation assay (scale bar = 100 μm). H Flow cytometric analysis of apoptosis. I Transwell migration assay (normalized to miR-NC + vector, scale bar = 100 μm). J Transwell invasion assay (normalized to miR-NC + vector, scale bar = 100 μm). The data are presented as the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001