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Fig. 5 | Molecular Cancer

Fig. 5

From: Long non-coding RNA NORAD/miR-224-3p/MTDH axis contributes to CDDP resistance of esophageal squamous cell carcinoma by promoting nuclear accumulation of β-catenin

Fig. 5

The NORAD/miR-224-3p/MTDH axis promotes CDDP resistance in ESCC cells by promoting nuclear accumulation of β-catenin. a The effects of NORAD/miR-224-3p/MTDH axis on the colony formation ability of KYSE30/CDDP-R and KYSE30 cells in the presence of CDDP. b The effects of NORAD/miR-224-3p/MTDH axis on CDDP induced apoptosis of KYSE30/CDDP-R and KYSE30 cells. c NORAD knockdown facilitates CDDP to induce cell cycle arrest in KYSE30/CDDP-R cells while miR-224-3p inhibitor rescued the arrest. d Overexpression of NORAD suppresses CDDP-induced cell cycle arrest in KYSE30 cells while miR-224-3p mimic neutralizes the suppression. e The effects of NORAD/miR-224-3p/MTDH axis on the expression and phosphorylation of MAPK, Akt, NF-κB and β-catenin in KYSE30/CDDP-R cells. f NORAD knockdown decreases the nuclear expression of MTDH in KYSE30/CDDP-R cells, detected by western blotting. g NORAD knockdown decreases the nuclear expression of MTDH in KYSE30/CDDP-R cells, detected by immunofluorescence. In all cases, error bars denote SD of triplicates. **P < 0.01, ***P < 0.001

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