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Fig. 1 | Molecular Cancer

Fig. 1

From: LncRNA PKMYT1AR promotes cancer stem cell maintenance in non-small cell lung cancer via activating Wnt signaling pathway

Fig. 1

LncRNA PKMYT1AR is highly expressed in NSCLC. a LncRNA PKMYT1AR was identified by integrative analysis using GEO datasets, GSE81089 (Blue): Next Generation Sequencing (RNAseq) from NSCLC, GSE144520 (Red): whole-transcriptome sequencing of A549 cells and cisplatin-resistant A549/DPP cells, GSE157427 (Green): gene expression profile for lung cancer stem cells. b The relative expression level of PKMYT1AR in fresh paired tissues isolated from NSCLC patients using Real-time RT-PCR assay, n=24. c The expression of PKMYT1AR in paired NSCLC peripheral blood serum examined by the Real-time RT-PCR assay, n=30. d The relative expression level of PKMYT1AR in TCGA-LUAD (adenocarcinoma; Normal: 59; Tumor: 533) and TCGA-LUSC (squamous cell carcinoma; Normal: 49; Tumor: 502), respectively. e The relative expression pattern of PKMYT1AR in GSE81089 dataset (Normal: 19, Tumor: 197). f The relative expression level of PKMYT1AR in NSCLC cancerous cell lines, including H358, H1975, H1299, H1650, A549 and SPC-A1 examined by Real-time RT-PCR, compared to normal human bronchial epithelial cell line: BEAS-2B. g PKMYT1AR high expression correlates with worse survival rate. h The ROC curve for PKMYT1AR (AUC=0.719) in LUAD using TCGA dataset. i The subcellular localization of PKMYT1AR was predicted by LncLocater. j PKMYT1AR was majorly localized in the cytoplasm of A549 cells using nuclear and cytoplasmic RNA fractionation assay followed by Real-time RT-PCR. β-actin and U1 expressions were used as cytoplasmic and nuclear fraction controls, respectively. k The sub-cellular localization of PKMYT1AR was examined by FISH. The nuclei were stained with DAPI (blue), and the 18S RNA was used as cytoplasm-localized RNA control. Scale bar=25μm. l The YY1 binding motif within the PKMYT1AR promoter region was predicted using JASPAR. m The correlation between YY1 and PKMYT1AR were examined in TCGA-LUAD dataset. n Depletion of YY1 reduced PKMYT1AR expression in A549 cells examined by the Real-time RT-PCR assay. * P < 0.05, ** P < 0.01, *** P < 0.001. MYT1AR=PKMYT1AR

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