Fig. 9

PKMYT1AR targeting ASOs impede tumor growth. a The relative expressions of PKMYT1AR transcripts were examined by Real-time RT-PCR after indicated ASO transfection. b Knockdown of PKMYT1AR inhibited cell proliferation examined by growth curve assays. c-d PKMYT1AR targeting ASOs repressed cell growth by colony formation assay. d is the quantification data for (c). e-f PKMYT1AR targeting ASOs repressed cell migration by trans-well assay (e). f is the quantification data for e. Scale bar=50 μm. g-h PKMYT1AR targeting ASOs inhibited cancer stem cell self-renewal ability by tumor-sphere formation assay (g). h is the quantification data for (g). Scale bar=50 μm. i PKMYT1AR targeting ASOs inhibited cancer stem cell marker genes expressions examined by immunoblot with indicated antibodies. j Schematic view of xenograft mouse model treated by indicated ASOs. k-m Representative xenograft tumor images (k), tumor masses (l) and tumor volumes (m) were shown for indicated groups treated by indicated ASOs. A549 cells were used. n-o Representative IHC staining of PKMYT1, Ki67 and CC3 (n) for indicated xenograft tumors. o is the quantification data for (n). Scale bar=50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001. ASO NC=ASO control