Fig. 2

Characterization of circLPAR1 in colorectal cancer tissues. A Schematic illustration of circLPAR1 formation through the circularization of exons 3 and 4 in LPAR1. The back-splice junction sequences were confirmed by Sanger sequencing. B Relative expression of LPAR1 mRNA and circLPAR1 after RNase R digestion. C Representative fluorescence images of circLPAR1 (green) in colorectal cancer tissues and NATs, as obtained by FISH. Scale bar, 250 μm (top) and 50 μm (bottom). D The relative expression of circLPAR1 in 79 pairs of colorectal cancer tissues and NATs measured by FISH. Statistical significance was assessed using two-tailed paired Student’s t-test. E The cellular localization of circLPAR1 in colorectal cancer tissue as determined by FISH. Scale bar, 50 μm; white arrowhead indicates circLPAR1 cellular localization. F The association of circLPAR1 expression level and overall survival in colorectal cancer patients. Kaplan-Meier analysis was used to plot and calculate the P value, and Cox regression was used to evaluate HR and 95% CI. ^*P < 0.05