Fig. 5

The effect of targeting exosomal cricLPAR1 in vivo. DLD1 cell lines transfected with circLPAR1/NC lentiviral vector were injected into humanized NCG mice, which were designated circLPAR1 and NC, and then DiR-labelled circLPAR1/NC-Exos were injected into NC mice, which were designated circLPAR1-Exos and NC-Exos. A Schematic diagram showing the process of establishing the mouse model of exosomal circLPAR1. B Harvested tumor tissues in the NC, circLPAR1, circLPAR1-Exos, and NC-Exos groups. The average tumor weight (C) and the mean tumor volumes (D) in the NC, circLPAR1, circLPAR1-Exos, and NC-Exos groups. E Representative images of mice after injection of DiR-labelled exosomes in the NC, circLPAR1, circLPAR1-Exos, and NC-Exos groups. Luminescence intensity ranges from low (blue) to high (red). F Representative images of Ki67 and BRD4 immunohistochemistry results. Scale bar, 50 μm. G The levels of Ki67 and BRD4 in immunohistochemistry staining. H Schematic diagram illustrating that exosomal circLPAR1 acted as a sponge of eIF3h to reduce the METTL3-eIF3h interaction, which inhibited the translation of BRD4, inducing suppression of colorectal cancer tumorigenesis. Statistical significance was assessed using two-tailed Student’s t-test. The values represent mean ± SD. ^*P < 0.05