Fig. 1

Single-cell enrichment of stemness/EMT gene signatures in sorafenib resistant cells. A IC50 of Huh7 parental (Huh7-P) and sorafenib-resistant (Huh7-R) cells. B-C Heatmaps showing the expression profiles of stemness/EMT (B) and Notch signalling related genes (C) as determined by single cell-RNA seq analysis. D Scatter plot showing the single-cell expression levels of Notch1–3 receptors in Huh7-P and Huh7-R cells. E t-SNE plots along with single-cell expression levels indicated on a purple/grey colour scale of cell-cycle related genes. The black circle represents the Huh7-R cell population with low gene expression. F tSNE plot representing the distribution of Huh7-P (red) and Huh7-R (green) cells. Arrows indicate a few Huh7-P cells (candidate stem cells) that are clustered together with Huh7-R cells. G Dot-plot showing the expression levels of stemness/EMT genes in Huh7-P candidate stem cells and Huh7-R cells