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Fig. 4 | Molecular Cancer

Fig. 4

From: circCDYL2 promotes trastuzumab resistance via sustaining HER2 downstream signaling in breast cancer

Fig. 4

circCDYL2 interacts with GRB7 protein in HER2+ BC cells. A Location of circCDYL2 in both BT474 and SK-BR-3 cells by FISH detection. Scale bar 50 μm. B qRT-PCR analysis of circCDYL2 in the cytoplasm and nuclear separated by PARIS kit. C qRT-PCR and Western Blot analysis of HER2 in BT474 and SK-BR-3 cells after silencing and over-expressing circCDYL2. D qRT-PCR analysis of circCDYL2 in both BT474 and SK-BR-3 after silencing HER2. E Mass spectrum of GRB7 protein. F Biotinylated circCDYL2 probe pull down results for GRB7 protein by Western Blot. G qRT-PCR analysis of circCDYL2 in RNA sample after RIP assay by anti-GRB7antibody. H Total and phosphorylased of AKT and ERK1/2 expression in both P-BT474 and P-SK-BR-3 cells, as detected by Western Blot after co-transfection with circCDYL2-overexpressing plasmid or GRB7 siRNA with Trastuzumab. I The proliferation of P-BT474 and P-SK-BR-3 cells after co-transfection circCDYL2-overexpressing plasmid or GRB7 siRNA with trastuzumab, as detected by CCK8 assay

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