Fig. 5
From: circCDYL2 promotes trastuzumab resistance via sustaining HER2 downstream signaling in breast cancer
circCDYL2 modulated GRB7 protein stability by Pin1. A, B GRB7 protein expression in BT474 and SK-BR-3 cells after silencing and overexpression circCDYL2 by Immunofluorescence (A) and Western Blot (B). C Western Blot of GRB7 protein in BT474 and SK-BR-3 cells after circCDYL2 over-expressing by over-expressing lentivirus treated with CHX (10 μM) at indicated time points. EV: empty vector; p-circ: over-expressing circCDYL2. D SK-BR-3 cells after circCDYL2 overexpression were subject to IP with anti-GRB7 antibody followed by Western Blot. E Western Blot of GRB7 protein in BT474 and SK-BR-3 cells after knock downing pin1 treated with CHX (10 μM) at indicated time points. F SK-BR-3 cells after knock downing pin1 were subject to IP with anti-GRB7 antibody followed by Western Blot. G SK-BR-3 cells after circCDYL2 overexpression were subject to IP with anti-GRB7 antibody or anti-pin1 followed by Western Blot. H GRB7 protein expression in SK-BR-3 cells after overexpression pin1 with or without overexpressing circCDYL2 by western blot