Fig. 6
From: circCDYL2 promotes trastuzumab resistance via sustaining HER2 downstream signaling in breast cancer
circCDYL2 enhances the formation of circCDYL2-GRB7-FAK complex. A Mass spectrogram of FAK protein. B Biotinylated circCDYL2 probe pull down results for FAK protein by Western Blot. C qRT-PCR analysis of circCDYL2 in RNA sample after RIP assay by anti-FAK antibody. D SK-BR-3 cells after circCDYL2 overexpression were subject to IP with anti-GRB7 antibody or anti-FAK followed by Western Blot. E SK-BR-3 cells after circCDYL2 silencing were subject to IP with anti-GRB7 antibody or anti-FAK followed by Western Blot. F Total and phosphorylased of AKT and ERK1/2 expression in both P-BT474 and P-SK-BR-3 cells, as detected by Western Blot after co-transfection with circCDYL2-overexpressing plasmid or FAK siRNA with Trastuzumab. G The proliferation of P-BT474 and P-SK-BR-3 cells after co-transfection circCDYL2-overexpressing plasmid or FAK siRNA with Trastuzumab, as detected by CCK8 assay