Fig. 3

Identification of murine lncRNA-IUR1. A A schematic representation of the genomic location of murine lncRNA-IUR1 and its adjacent genes P2ry2 and Fchsd2. The orientation of arrows indicated the transcription direction. B RT-PCR (left) and quantitative real-time PCR (right) were performed to detect the expression of murine lncRNA-IUR1 in NS2 cells treated with imatinib. Data of quantitative real-time PCR (right) are presented as mean ± SEM. n = 3, **p < 0.01, ***p < 0.001. C Quantitative real-time PCR analysis of the RNA levels of murine lncRNA-IUR1 in W44 cells treated with imatinib. Data are presented as mean ± SEM. n = 3, ***p < 0.001. D Agarose gel analysis of 5′ RACE and 3′ RACE PCR products of murine lncRNA-IUR1. 5′ and 3′ end sequences were show in below. E Murine lncRNA-IUR1 was cloned into pNL vector with an N-terminal Flag tag in three reading frames. The constructs were transfected into HEK293T cells for 48 h. Cell lysates were harvested and subjected to Western blotting with Flag antibody. Flag-KU70 was served as a positive control