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Fig. 4 | Molecular Cancer

Fig. 4

From: A novel imatinib-upregulated long noncoding RNA plays a critical role in inhibition of tumor growth induced by Abl oncogenes

Fig. 4

Altering murine lncRNA-IUR1 expression regulates v-Abl-transformed cell survival in vitro and tumor growth in vivo. A, B Control and lncRNA-IUR1 knockdown NS2 cells were generated. NS2 cells were infected with GFP-positive lentiviruses harboring control (sh-luc) or murine lncRNA-IUR1 shRNA (sh-mIUR1) respectively. GFP-positive NS2 cells were sorted by FACS. Shown were representative micrographs of the generated cells (A). RT-PCR was performed to examine the expression of lncRNA-IUR1 in NS2 cells stably expressing sh-luc or sh-mIUR1 with or without imatinib treatment (B). C Cell viability of control and lncRNA-IUR1 knockdown NS2 cells was analyzed by flow cytometry upon treatment with imatinib (2.5 μM). Data are presented as mean ± SEM. n = 3, *p < 0.05, **p < 0.01. D Nude mice were subcutaneously injected with control or murine lncRNA-IUR1 knockdown NS2 cells. Tumor growth was measured by bioluminescent imaging. Shown were representative images from at least three independent experiments. E RT-PCR was performed to examine the expression of lncRNA-IUR1 in NS2 cells stably expressing empty vector (EV) or murine lncRNA-IUR1 (mIUR1). F Cell survival analysis of control and lncRNA-IUR1 overexpressing NS2 cells in response to imatinib treatment (2.5 μM). Data are presented as mean ± SEM. n = 3, *p < 0.05. G, H Nude mice were subcutaneously injected with control or lncRNA-IUR1 overexpressing NS2 cells. Tumor growth was monitored. Shown were representative images from at least three independent experiments (G). The expression of lncRNA-IUR1 in tumors fromed by control or lncRNA-IUR1 overexpressing NS2 cells, was analyzed by quantitative real-time PCR (H). Data (H) are presented as mean ± SEM. n = 3, ***p < 0.001

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