Fig. 6

Knockout of murine lncRNA-IUR1 in mice facilitates Bcr-Abl-mediated transformation of primary bone marrow cells, Abl-transformed cell survival and the development of leukemia in mice. A The photo of lncRNA-IUR1 knockout (KO) mice and their WT littermate. B RT-PCR was performed to examine the expression of lncRNA-mIUR1 in the spleen, bone marrow cells (BMCs), thymus and white blood cells (WBCs) from WT or lncRNA-mIUR1 KO mice. C Primary bone marrow cells derived from WT or lncRNA-IUR1 KO mice, were infected with the retrovirus carrying Bcr-Abl oncogene, and the efficiency of transformation was measured by counting the number of Bcr-Abl-transformed cell clones. Data are presented as mean ± SEM. n = 3, ***p < 0.001. D–F Sub-lethally irradiated lncRNA-IUR1 KO and WT mice were injected with GFP-positive NS2 cells, or equal volume of PBS. The body weight of indicated mice was monitored for a period of 12 days (D). Quantity of WBCs (E) and RBCs (F) in peripheral blood of indicated mice, was detected by blood routine examination. G Bioluminescent imaging analysis of the distribution of GFP-positive NS2 cells in sub-lethally irradiated lncRNA-IUR1 KO and WT mice at the 12th day after in vivo leukemia transplantation. PBS group was the negative control. Shown were representative images from at least three independent experiments with similar results. H Shown were representative images of spleens from indicated mice at the 12th day after in vivo leukemia transplantation