Fig. 7

LncRNA-IUR1 negatively regulates STAT5-mediated GATA3 expression in Abl-positive leukemic cells. A Transcriptome RNA sequencing analysis of control (sh-luc) and lncRNA-IUR1 knockdown (sh-IUR1) K562 cells. B, C Quantitative real-time PCR analysis of GATA3 mRNA levels in control and lncRNA-IUR1 knockdown K562 (B) or NS2 (C) cells. Data are presented as mean ± SEM. n = 3, *p < 0.05, **p < 0.01. D Quantitative real-time PCR was performed to examine GATA3 mRNA levels in white blood cells (WBCs) derived from lncRNA-IUR1 KO and WT mice. Data are presented as mean ± SEM. n = 3, *p < 0.05. E RT-PCR was performed to examine the expression of GATA3 and lncRNA-IUR1 in K562 cells treated with imatinib. F Quantitative real-time PCR analysis of GATA3 mRNA levels in control and STAT5 knockdown K562 cells. Data are presented as mean ± SEM. n = 3, *p < 0.05. G, H Phosphorylation levels of STAT5 were examined in control and lncRNA-IUR1 knockdown K562 (G) or NS2 (H) cells by Western Blotting. I Western Blotting was performed to detect phosphorylation levels of STAT5 in control and lncRNA-IUR1 knockdown NS2 cells treated with STAT5 inhibitor