Fig. 8

LncRNA-IUR1 dampens Abl-induced tumorigenesis by suppressing GATA3 expression. A RT-PCR was performed to examine GATA3 mRNA levels in NS2 cells stably expressing control (sh-luc) or GATA3 shRNA (sh-GATA3). B Cell viability of control and GATA3 knockdown NS2 cells, was analyzed by flow cytometry upon treatment with imatinib (2.5 μM). Data are presented as mean ± SEM. n = 3, **p < 0.01. C Nude mice were subcutaneously injected with control or GATA3 knockdown NS2 cells. Tumor growth was measured by bioluminescent imaging. Shown were representative images from at least three independent experiments. D RT-PCR was performed to examine lncRNA-IUR1 and GATA3 RNA levels in K562 cells stably expressing empty vector (EV), lncRNA-IUR1, or lncRNA-IUR1 and GATA3. E Cell survival analysis of K562 cells stably expressing EV, lncRNA-IUR1, or lncRNA-IUR1 and GATA3 in response to imatinib treatment (5 μM). Data are presented as mean ± SEM. n = 3, **p < 0.01. (F) Nude mice were subcutaneously injected with K562 cells stably expressing EV, lncRNA-IUR1, or lncRNA-IUR1 and GATA3. Shown were representative images of tumors excised from these nude mice