Fig. 6

The RNA-binding protein IGF2BP3 binds to hsa_circ_0003258 in PCa cells. A Venn diagram showing the predicted RNA binding proteins. B The binding sites between hsa_circ_0003258 and IGF2BP3 were predicted online. C The interaction possibility of hsa_circ_0003258 and IGF2BP3 detected in RPISeq and the result showed that hsa_circ_0003258 bound to IGF2BP3. D Western blot analysis of IGF2BP3 levels in pulldown assays using a biotinylated antisense oligomer targeting the junction of hsa_circ_0003258. E RIP assays showing the association between IGF2BP3 and Hsa_circ_0003258. Top, IP efficiency of IGF2BP3-antibody shown in Western blotting. Bottom, relative enrichment representing RNA levels associated with IGF2BP3 relative to an input control. IgG antibody served as a control. F IF and FISH assay showing that hsa_circ_0003258 is colocalized with IGF2BP3 protein in the cytoplasm. G Western blot showing protein levels of IGF2BP3 after silencing of hsa_circ_0003258. H m6A bound to hsa_circ_0003258 performed by meRIP. * P < 0.05; *** P < 0.001