Fig. 2From: CircNFIB inhibits tumor growth and metastasis through suppressing MEK1/ERK signaling in intrahepatic cholangiocarcinomacNFIB suppresses proliferation and metastasis of ICC cells in vitro. (A) Cell Counting Kit-8 (CCK-8) assays showed that cNFIB inhibited the proliferation of ICC cells. (B) EdU immunofluorescence assays to detect the effects of cNFIB on the capacity of DNA duplication of ICC cells . (C) Wound healing showed that cNFIB suppressed the migration of ICC cells. (D) Effects of cNFIB on cell migration and invasion examined by transwell assays. Data were shown as mean ± SD, unpaired Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars, 100 μm. Abbreviations: NC, normal control; OE, overexpressionBack to article page