Fig. 3

cNFIB suppresses ICC growth and metastasis in vivo. (A) and (B) Subcutaneous xenografts dissected from nude mice inoculated with the indicated cNFIB-knockdown HuCCT1 cells or cNFIB-overexpression RBE cells, n = 5. (C) and (D) The volume of subcutaneous xenograft tumors of indicated cells isolated from nude mice, n = 5. (E) and (G) Left, representative bioluminescent images of liver tumors from orthotopic-implantation models inoculated with cNFIB-knockdown HuCCT1 cells or cNFIB-overexpression RBE cells. Right, statistical analysis of total liver photon flux emitted from the nude mice, n = 5. (F) and (H) Representative fluorescent images (left) and hematoxylin-eosin (HE) staining of metastatic nodules (right) of liver orthotopic-implantation models inoculated with indicated RBE and HuCCT1 cells. The number of metastatic foci formed in the livers was indicated in the bar graph, n = 5. (I) and (K) Left, representative bioluminescent images of lung metastatic foci from nude mice after injection of indicated RBE and HuCCT1 cells through tail vein. Right, statistical analysis of total lung photon flux emitted from the mice, n = 5. (J) and (L) Representative fluorescent images (left) and HE staining of metastatic nodules (right) of lung metastasis models from indicated RBE and HuCCT1 cells. The number of metastatic foci formed in the lungs was indicated in the bar graph, n = 5. Data were shown as mean ± SEM, unpaired Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.001