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Fig. 1 | Molecular Cancer

Fig. 1

From: EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3

Fig. 1

CircRNA expression profiles in glioma and characterization of circNEIL3. A Volcano plots of circRNAs that were differentially expressed between LGG tissues and NBTs. B Volcano plots of circRNAs that were differentially expressed between GBM samples and LGG samples. C Venn diagram showing the overlapping upregulated circRNAs (Log2 FC > 4) between LGG tissues and NBTs and between GBM and LGG tissues. D Relative expression of circNEIL3 in NBTs, LGG tissues and GBM tissues detected by high-throughput sequencing. E An ROC curve was used to assess the diagnostic value of circNEIL3 for glioma. F Schematic illustration of the genomic location and back splicing of circNEIL3 with the splicing site validated by Sanger sequencing. G RT-PCR with divergent and convergent primers and agarose gel electrophoresis analysis were performed to detect the presence of circNEIL3 and its maternal gene NEIL3 in cDNA and gDNA samples from GBM cells. H Actinomycin D treatment was used to evaluate the stability of circNEIL3 and NEIL3 mRNA in GBM cells. I RNase treatment was used to evaluate the stability of circNEIL3 and NEIL3 mRNA in GBM cells. J Nuclear-cytoplasmic fractionation assays showed that circNEIL3 was mostly localized in the cytoplasm of GBM cells. K RNA FISH assays showing the cellular localization of circNEIL3 in GBM cells. The circNEIL3 probe was labelled with Cy3 (red), while nuclei were stained with DAPI (blue). Scale bar, 25 μm. All data are presented as the means ± SD, ns, P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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