Fig. 2

CircNEIL3 promotes proliferation and metastasis of GBM cells in vitro and vivo. CCK8 assays showing the proliferation ability of GBM cells transfected with (A) sh-NC or sh-circNEIL3, and (B) ov-NC or ov-NEIL3, n = 3. Representative transwell migration and invasion assays showing the migration and invasion ability of GBM cells transfected with (C) sh-NC or sh-circNEIL3, and (D) ov-NC or ov-NEIL3. Quantification histogram represented relative cell numbers, n = 3, scale bar, 200um. E Up, bioluminescent image showing the tumor size for animals in different groups in the indicated time. n = 5 for each group. Down, statistical analysis of bioluminescent tracking plots. F Kaplan-Meier survival curves for animals in different groups, n = 5 for each group. G Represented CD44 and KI67 immunohistochemistry images for a subgroup of animal sacrificed simultaneously in each group, n = 5 for each group, scale bar, 20um. All data are presented as the means ± SD, **p < 0.01, ***p < 0.001, ****p < 0.0001