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Fig. 5 | Molecular Cancer

Fig. 5

From: EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3

Fig. 5

CircNEIL3 blocked the binding between IGF2BP3 and HECTD4. A CCk8 assays showing the proliferation ability of GBM cells transfected with si-NC or si-HECTD4. n = 3. b. Representative transwell migration and invasion assays showing the migration and invasion ability of GBM cells transfected with si-NC or si-HECTD4. Quantification histogram represented relative cell numbers, n = 3, scale bar, 200 μm. C IF assays showing the co-localization of IGF2BP3 (green) with HECTD4 (red) in GBM cells. Scale bar, 25 μm. D Western blot assays showing the expression of HECTD4 and IGF2BP3 in GBM cells transfected with si-NC or si-HECTD4. E Co-IP assays showing the ub modification level in GBM cells transfected with si-NC or si-HECTD4. F Co-IP assays showing the binding between HECTD4 with IGF2BP3 proteins in GBM cells transfected with ov-NC or ov-circNEIL3. G Co-IP and western blot assays showing the interaction between HECTD4 and different IGF2BP3 domain in in 293 T cells transfected with vectors expressing the Flag-tagged FL or the truncated mutants (RRM1–2, KH1–2, KH3–4), using Flag antibodies. All data are presented as the means ± SD, **P < 0.01, ***P < 0.001, ****P < 0.0001

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