Fig. 6

CircNEIL3 facilitates macrophages infiltration in glioma. A The abundance of each TME infiltrating cells and regulators in circNEIL3 high and low groups. The statistical p-value was calculated using the nonparametric Wilcoxon test. Representative transwell migration assays showed the chemotaxis ability of human THP1-differentiated macrophages by exposing them to conditioned medium (CM) from GBM cells transfected with (B) ov-NC or ov-circNEIL3, and (C) sh-NC or sh-circNEIL3. Quantification histogram represented relative cell numbers, n = 3, scale bar, 200um. GSEA of CONRDENONsi YAP Conserved signature showed that glioma samples with high (D) circNEIL3 in our local glioma dataset, and (E) IGF2BP3 expression in TCGA glioma dataset were enriched in the YAP1 signaling compared to glioma samples with low expression, respectively. NES, normalized enrichment score; FDR, false discovery rate. F qRT-PCR assays showing the relative mRNA expression of CCL2 and LOX in GBM cells transfected with (left) sh-NC or sh-circNEIL3, and (right) ov-NC or ov-circNEIL3, n = 3. G qRT-PCR assays showing the relative mRNA expression of CCL2 and LOX in GBM cells co-transfected with ov-NC, ov-circNEIL3 and sh-IGF2BP3 as indicated, n = 3, and the ov-circNEIL3 group is indicated as the control. Western blot assays showing the protein expression of YAP1 and LOX in GBM cells (H) transfected with sh-NC or sh-circNEIL3, and (I) co-transfected with ov-NC, ov-circNEIL3 and sh-IGF2BP3 as indicated. All data are presented as the means ± SD, ns, P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001