Fig. 2

LncRNA TUG1-miR-328-3p-SRSF9 mRNA axis may be involved into tumor growth in vivo and may be effectively regulated by the anti-HCC prescription FBRP. A Pharmacological effects of FBRP on pathological changes into the liver tissues of the nude mice with carcinoma in situ. B Pharmacological effects of FBRP on the histological changes into liver tissues of the in subcutaneous xenograft HCC nude mouse model. Boxes represent the area with typical pathological changes. Green arrows represent the HCC cells. Red arrows represent the necrotic cells among HCC cells. Blue arrows represent massive infiltration of inflammatory cells. C Generation of neoplastic lesions of nude mice in different groups. D, E, F Expression and subcellular localization of SRSF9 protein in liver tissues of nude mice and the quantification results examined by immunohistochemistry. Quantification, localization and co-localization of miR-328-3p and lncRNA TUG1 were detected by RNA FISH assay. Data were presented as the mean ± sem. From three independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, comparison with the CON group; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001, comparison with the model group. G The expression levels of SRSF9 mRNA, miR-328-3p and lncRNA TUG1 in liver tissues of DEN-induced “Hepatitis-Liver Fibrosis-Liver Cancer” malignant transformation rats and FBRP intervention rats. Data were presented as the mean ± sem. From three independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, comparison with the CON group; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001, comparison with the DEN group