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Fig. 6 | Molecular Cancer

Fig. 6

From: circPTEN1, a circular RNA generated from PTEN, suppresses cancer progression through inhibition of TGF-β/Smad signaling

Fig. 6

circPTEN1 suppresses CRC metastasis by inhibiting TGF-β/Smad-mediated EMT. A. The expression of Snail, Slug and ZEB1 in circPTEN1 knockdown or overexpressed LoVo cells as indicated in Supplementary Fig. 6C and Supplementary Fig. 6E treated with TGF-β1 was analyzed by qRT-PCR. RS: pLCDH-circPTEN1-RS. **, p < 0.01. ***, p < 0.001. ****, p < 0.0001. B. Dual-luciferase reporter assays of LoVo cells as indicated in Supplementary Fig. 6C and Supplementary Fig. 6E transfected with Snail, Slug or ZEB1 promoters in the presence or absence of TGF-β1 for 24 h. RS: pLCDH-circPTEN1-RS. C. LoVo cells, as indicated in Supplementary Fig. 6C and Supplementary Fig. 6E were treated with TGF-β1 for 24 h. The expression of E-cadherin, N-cadherin and vimentin was determined by immunoblot analysis. RS: pLCDH-circPTEN1-RS. D. circPTEN1 knockdown LoVo cells, circPTEN1 knockdown LoVo cells overexpressing Ski, or control cells were treated with TGF-β1 for 24 h. The expression of E-cadherin, N-cadherin, vimentin and Ski was determined by immunoblot analysis. E. circPTEN1 knockdown LoVo cells, circPTEN1 knockdown LoVo cells overexpressing Ski, or control cells were treated with TGF-β1 for 48 h, and the motility of these cells was sequentially evaluated through Transwell migration assays and invasion assays. Scale bar, 40 μm. F. Migrated cells in Fig. 6E were counted in five random fields per well to calculate cell migration and invasion ability. ****, p < 0.0001

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