Fig. 6

The C allele at SNP rs8570 is associated with several parameters of more aggressive disease in CLL patients. a Schematic representation of the rs8570 location in the 3’UTR of RRAS2 mRNA. b Representative examples of the Sanger sequencing chromatograms showing the 3 possibilities of allele dosage at the position of rs8570. The rs8570 position is highlighted by a red rectangle. c Classification of patient samples according to the genotype at SNP rs8570 detected by a dedicated RT-qPCR strategy using specific primers for each of the 2 alleles at that position. The patients sequenced as GG, GC or CC with Sanger sequencing are plotted with dark blue, purple and orange dots, respectively. d Pie chart representation of the Observed versus the Expected distribution of GG, GC and CC genotypes at position of the SNP rs8570 in our study cohort of CLL patients. e Box and whiskers plots showing all points and median value of RT-qPCR RRAS2 mRNA expression in blood from all patients in our study cohort classified according to GG, GC or CC genotype at position of the SNP rs8570. Ttwo-tailed unpaired t-test with Welch’s correction. f Box and whiskers plots showing all points and median value of RT-qPCR RRAS2 mRNA expression in blood from all patients in our study cohort harboring none (GG) or at least one (GC and CC) 124C at position of the SNP rs8570. Two-tailed unpaired t-test with Welch’s correction. g Box and whiskers plots showing all points and median value of total lymphocyte count in the blood of patients harboring two G alleles or at least one C. Two-tailed unpaired t-test with Welch’s correction. h Box and whiskers plots showing all points and median value of the percentage of CD19+ B cells in the blood of patients harboring two G alleles or at least one C. Two-tailed unpaired t-test with Welch’s correction. i Box and whiskers plots showing all points and median value of the percentage of CD19 + CD5+ leukemic B cells in the blood of patients harboring two G alleles or at least one C. Two-tailed unpaired t-test with Welch’s correction. j Box and whiskers plots showing all points and median value of the platelet count in the blood of patients harboring two G alleles or at least one C. Two-tailed unpaired t-test with Welch’s correction. k Contigency test of the distribution of patients diagnosed as having MBL or CLL according to a GG or GC + CC genotype. l Contigency test of the distribution of all samples in our study cohort according to presenting or not chromosomal alterations by FISH and a GG or GC + CC genotype. m Contigency test of the distribution of samples in our study cohort according to presenting normal caryotype or presenting deletions in chromosome 11q or in chromosome 17p (or both) and a GG or GC + CC genotype. n Contingency test of the distribution of all samples in our study cohort according to presenting or not mutated IgHV and a GG or GC genotype. o Contingency test of the distribution of all samples in our study cohort according to male or female sex and a GG or GC + CC genotype