Fig. 2

Knockdown of circROBO1 inhibits proliferation, migration, and invasion of BC cells and overexpression of circROBO1 elicits the opposite effects. a The schematic illustration of circROBO1 overexpression vector and knockdown of circROBO1 via siRNAs. b RT-qPCR was performed to measure the efficiency of overexpression and knockdown of circROBO. c Edu assays of BC cells was conducted to evaluate proliferation (Scale bar=50μm). d Colony formation was performed to evaluate the cell proliferation ability. e CCK8 assays were used for analyzing the growth curves of cells. f and g Wound healing assays and Transwell migration, invasion assays (Scale bar=100μm) were performed to measure the migration and invasion abilities in BC cells. The data are showed as the mean ± SD, *P < 0.05 **P < 0.01, ***P < 0.001 and all above experiments have been repeated for three times