Fig. 5

CircROBO1 functions as a sponge of miR-217-5p. a Venn diagram showed the intersection of TargetScan and Circinteractome in potential miRNA binding sites of circROBO1. The two potential binding sites of miR-217-5p in circROBO1 was displayed. b According to the TCGA database, miR-217 was downregulated in BC and indicated a favorable prognosis in BC. c RT-qPCR was performed in 51 pairs of BC tissues which validated there existed a negative correlation between miR-217-5p and circROBO1. d Knockdown or overexpression of circROBO1 upregulated or downregulated miR-217-5p by RT-qPCR. e RNA pull-down assays with a biotin-labeled probe of junction site sequences of circROBO1 were performed in BT-549 to validate the interaction between circROBO1 and miR-217-5p and the enrichment were detected by RT-qPCR to test the enrichment of miR-217-5p. f Schematic illustration displayed the dual-luciferase report vectors with wild-type (WT) and mutant-type (MT) miR-217-5p binding sites. g The relative activities of luciferase were detected after co-transfection of circROBO1-WT or circROBO1-MT and miR-217-5p mimics or inhibitors with control respectively in 293T cells. h-j The cell viability of BC cells were detected after co-transfection with indicated siRNAs, vectors, miRNAs or inhibitors by colony formation assays, Edu assays (Scale bar=50μm), and CCK8 assays respectively. k and l The migration and invasion abilities of BC cells co-transfected with indicated siRNAs, vectors, miRNAs or inhibitors were measured by transwell migration and invasion assays (Scale bar=100μm) and wound healing assays respectively. The data are showed as the mean ± SD, *P < 0.05 **P < 0.01, ***P < 0.001 and all above experiments have been repeated for three times