Fig. 2

circFARP1 is critical for CAFs to induce GEM resistance in PDAC cells. Panc-1 and MiaPaCa-2 cells were grown in conditioned medium (CM) from CAFs stably transfected with empty vector, circFARP1, lenti-NC-shRNA, or lenti-circFARP1-shRNA for 2 weeks and then subjected to the indicated experiments. A-B GEM IC50 of Panc-1 and MiaPaCa-2 determined by constructing a dose–response curve. C-D Cell proliferation of Panc-1 and MiaPaCa-2 cells was detected by CCK-8 assay with the indicated treatment. E-F Colony formation assays of Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). G-H Spheroid growth assay of Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). Scale bars, 50 μm. I-J Flow cytometry analysis of GEM-induced (10 μM) apoptosis in Panc-1 and MiaPaCa-2 cells. Data are expressed as the mean ± SD. **p<0.01 and ***p<0.001