Fig. 5

circFARP1 enhances LIF secretion via CAV1 in CAFs. A-D Panc-1 and MiaPa-2 cells were grown in CM from CAFs transfected with lenti-NC-shRNA or lenti-CAV1-shRNA for 2 weeks and then subjected to subsequent experiments. A Cell viability of pretreated Panc-1 and MiaPaCa-2 cells was detected by CCK-8 assay. B Colony formation assays of Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). C Sphere forming assays of Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). Scale bars, 50μm. D Flow cytometry analysis of GEM-induced (10 μM) apoptosis in Panc-1 and MiaPaCa-2 cells. E-G The mRNA expression, protein level, and secretion level of LIF in CAFs with CAV1 silencing or not. n.s., no significant. H ELISA of the supernatant LIF level in CAFs transfected with lenti-circFARP1 and lenti-CAV1-shRNA alone or together. I-K Panc-1 and MiaPa-2 cells were grown in CM from CAFs transfected with lenti-circFARP1 and lenti-CAV1-shRNA alone or together for 2 weeks and then subjected to subsequent experiments. I The viability of Panc-1 and MiaPaCa-2 cells was detected by CCK-8 assay. J Representative images and quantification of sphere formation ability of Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). Scale bars,50μm. K Flow cytometry analysis of GEM-induced (10 μM) apoptosis in Panc-1 and MiaPaCa-2 cells. Data are expressed as the mean ± SD. **p<0.01, ***p<0.001