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Fig. 6 | Molecular Cancer

Fig. 6

From: circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis

Fig. 6

circFARP1 functions as a miR-660-3p sponge in CAFs. A qRT–PCR analysis of the enrichment of the indicated miRNAs in CAFs by RNA pull-down assay. B Schematic illustration showing the sequence alignment of circFARP1 with miR-660-3p. C The luciferase activities of the circFARP1 luciferase reporter plasmid (WT or MUT) following transfection with NC mimic or miR-660-3p mimics into CAFs. D The colocalization of circFARP1 and miR-660-3p was detected by FISH assay. Scale bar, 100μm. E-H Panc-1 and MiaPa-2 cells were grown in CM from CAFs transfected with miR-660-3p mimic or inhibitor for 2 weeks and then subjected to subsequent experiments. (E) CCK-8 assay of cell viability in Panc-1 and MiaPaCa-2 cells. (F) Representative images and quantification of colony formation in Panc-1 and MiaPaCa-2 cells treated with GEM (5 μM). (G) Representative images and quantification of sphere formation in Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). (H) Flow cytometry analysis of apoptosis in Panc-1 and MiaPaCa-2 cells treated with GEM (10 μM). Data are expressed as the mean ± SD. **p<0.01 and ***p<0.001

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