Fig. 7

circFARP1 enhances LIF expression via miR-660-3p. A-B qRT–PCR and western blot analysis of LIF expression in CAF1 and CAF2 cells transfected with miR-NC or miR-660-3p mimic. C-D qRT–PCR and western blot analysis of LIF expression in CAF1 and CAF2 cells transfected with NC or miR-660-3p inhibitor. E Top panel, schematic illustrating the sequence alignment of miR-660-3p with the 3’UTR of LIF. Bottom panel, the luciferase activities of the LIF-3’ UTR luciferase reporter plasmid containing wild-type (WT) and miR-660-3p binding site mutated (Mut) and transfected with the NC mimic or miR-660-3p mimic in CAFs. F-H qRT–PCR, western blot and ELISA analysis of LIF expression in CAFs transfected with circFARP1 and miR-660-3p mimic alone or together. I-K Panc-1 and MiaPa-2 cells were grown in CM from CAFs transfected with circFARP1 and miR-660-3p mimic alone or together and then subjected to subsequent experiments. I The viability of Panc-1 and MiaPaCa-2 cells was detected by CCK-8. J Representative images and quantification of sphere formation assays on Panc-1 and MiaPaCa-2 cells under GEM treatment (5 μM). K Flow cytometry analysis of apoptosis in Panc-1 and MiaPaCa-2 cells treated with GEM (10 μM). Data are expressed as the mean ± SD. **p<0.01 and ***p<0.001