Skip to main content
Fig. 1 | Molecular Cancer

Fig. 1

From: Splicing factor derived circular RNA circCAMSAP1 accelerates nasopharyngeal carcinoma tumorigenesis via a SERPINH1/c-Myc positive feedback loop

Fig. 1

circCAMSAP1 is highly expressed in NPC. A The expression of circCAMSAP1 was measured in 29 NPC tissues and 12 non-cancerous NPE tissue samples by RT-qPCR. β-actin was used as an internal reference. NPE, nasopharyngeal epithelium; NPC, nasopharyngeal carcinoma. ***, p < 0.001. B Representative images of circCAMSAP1 in 82 NPC tissues and 29 adjacent NPE tissues by in situ hybridization. × 200, scale bar = 50 μm; × 400, scale bar = 20 μm. The statistical data was shown in the right panel. ****, p < 0.0001. C The stability of circCAMSAP1 was detected in three RNase R-treated NPC cells by RT-qPCR. CAMSAP1 mRNA was used as a negative control. ***, p < 0.001; ****, p < 0.0001. D The stability of circCAMSAP1 was examined in NPC cells after treating with actinomycin D for 0, 6, 12 and 24 h. The relative RNA levels of circCAMSAP1 and CAMSAP1 mRNA in NPC cells were measured by RT-qPCR. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. E Intracellular localization of circCAMSAP1 (red) in three NPC cells, as determined by fluorescence in situ hybridization using a digoxigenin-labeled circCAMSAP1 probe. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. F The cellular localization of circCAMSAP1 was examined in three NPC cells by nuclear and cytoplasmic separation test. GAPDH and U6 were used as positive controls for cytoplasm and nucleus, respectively

Back to article page