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Fig. 4 | Molecular Cancer

Fig. 4

From: Splicing factor derived circular RNA circCAMSAP1 accelerates nasopharyngeal carcinoma tumorigenesis via a SERPINH1/c-Myc positive feedback loop

Fig. 4

circCAMSAP1 stabilizes the expression of SERPINH1 by binding to its 3’UTR. A Western blotting analysis of SERPINH1 expression in three NPC cell lines after overexpression and knockdown of circCAMSAP1. B RT-qPCR analysis of SERPINH1 expression in three NPC cell lines after overexpression and knockdown of circCAMSAP1. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. C The luciferase reporter gene activity was analyzed in three NPC cell lines after overexpression or knockdown of circCAMSAP1. The wild-type (WT) or mutant (MT) of the SERPINH1 3’UTR were constructed and transfected into NPC cells. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. D The circRIP assay was used to detect the binding of circCAMSAP1 to the 3’UTR of SERPINH1 mRNA. **, p < 0.01; ****, p < 0.0001. E The stability of SERPINH1 mRNA was measured in NPC cells after overexpression or knockdown of circCAMSAP1. Cells were treated with actinomycin D for 0, 60, 120 min and RT-qPCR was used to measure the expression of SERPINH1 mRNA. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

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