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Fig. 7 | Molecular Cancer

Fig. 7

From: Splicing factor derived circular RNA circCAMSAP1 accelerates nasopharyngeal carcinoma tumorigenesis via a SERPINH1/c-Myc positive feedback loop

Fig. 7

c-Myc combines with SRSF10 to promote the expression of circCAMSAP1. A The expression of CAMSAP1 pre-mRNA was examined by RT-qPCR in NPC cells after overexpression or knockdown of c-Myc. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. B The expression of circCAMSAP1 was examined by RT-qPCR in NPC cells after overexpression or knockdown of c-Myc. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. C The luciferase reporter gene activity of the CAMSAP1 promoter was analyzed in NPC cells after overexpression of c-Myc. ***, p < 0.001; ****, p < 0.0001. D The binding of transcription factor c-Myc to the CAMSAP1 promoter detected by ChIP assay. Two sites on the CAMSAP1 promoter were selected according to the c-Myc binding sites (site 1 and site 2) using the Jasper and PROMO software. A non-binding region (site 3) was used as a negative control. **, p < 0.01; ***, p < 0.001. E The expression of circCAMSAP1 was examined by RT-qPCR in NPC cells after overexpression or knockdown of SRSF10. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. F SRSF10 bound to the flanking intron sequence of circCAMSAP1 detected by RIP assay. Intron 1 and intron 3 were selected for RIP experiments using the SRSF10 antibody and referred to the SRSF10 binding site of CAMSAP1 pre-mRNA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. G The expression of SRSF10 was examined by RT-qPCR in NPC cells after knockdown of c-Myc. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. H The SRSF10 expression in NPC cells after knockdown of c-Myc examined by western blotting

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