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Fig. 3 | Molecular Cancer

Fig. 3

From: Tumor-suppressive circRHOBTB3 is excreted out of cells via exosome to sustain colorectal cancer cell fitness

Fig. 3

The biogenesis of circRHOBTB3 is regulated by a cis-element on its own sequence. (A) Schematic diagram of the genomic regions of RHOBTB3 exon 6 and exon 7 containing flanking Alu repeats and long introns. (B) Schematic diagram of the gRNA and homologous recombination donors. (C) RT-PCR and (D) RT–qPCR for CRISPR/Cas9-mediated genomic deletions in SW480 cells. (E) Cell migration, invasion, (F) and proliferation assays of circRHOBTB3-KO SW480 cells. The right column diagram shows the quantification analysis results. (G) Schematic diagram of truncated circRHOBTB3 vector construction and primer design for detection. (H) Circularization efficiency of 100 nt truncation and (I) further 25 nt truncation, normalized to FULL. All experiments were repeated for three times, data were shown as mean ± SD (D, F) or mean ± SEM (E, H, I), * P < 0.05, ** P < 0.01, *** P < 0.001, NS P > 0.05, in Student’s test (D, F) or paired Student’s test (E, H, I)

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