Table 2 Viral vectors for delivery of CRISPR/Cas9 system
From: Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
Delivery vehicle | Packaging capacity | Advantages | Disadvantages |
|---|---|---|---|
Adenovirus | Approximately 8-10 kb | Efficient delivery Large cargo size | Inflammatory response |
Adeno-associated virus (AAV) | Approximately 4.7 kb | Multiple serotypes Low immunogenicity Can transduce dividing and non-dividing cells in different tissues | Pre-existing neutralizing antibodies Long-term expression of Cas9 causing off-target effects |
Lentivirus | Approximately 10 kb | High transduction efficiency Large cargo size Low immunogenicity Can transduce dividing and non-dividing cells in different tissues | Non-specific DNA integration causing cancer risk Complex packaging structure |