Table 3 Nanotechnology-based delivery system for CRISPR/Cas9
From: Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
Delivery system | Cargo options | Advantages | Disadvantages |
|---|---|---|---|
Lipid nanoparticles | RNP plasmid DNA RNP complex Cas9 mRNA sgRNA Donor DNA | High biocompatibility Low immunogenicity Reduce off-target effects Can be mass produced Low cost | Degradation in vivo |
Polymer nanoparticles | RNP plasmid DNA RNP complex Cas9 mRNA sgRNA Donor DNA | High biocompatibility Low immunogenicity Reduce off-target effects Can be mass produced Low cost | Toxicity Limited delivery efficiency |
Golden nanoparticles | RNP plasmid DNA RNP complex Cas9 mRNA sgRNA Donor DNA | High biocompatibility Low immunogenicity Reduce off-target effects Can be mass produced Low cost | Limited delivery efficiency |