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Fig. 4 | Molecular Cancer

Fig. 4

From: Methyltransferase like 7B is a potential therapeutic target for reversing EGFR-TKIs resistance in lung adenocarcinoma

Fig. 4

METTL7B enhanced ROS scavenging through upregulation of antioxidant enzymes. a FLAG-NC and FLAG-METTL7B was stably transfected into TKIs-sensitive PC9 and HCC827 cells, and relative protein levels were measured by Western blot. b and c The enzymatic activities of GPX and SOD were detected by Cu/Zn-SOD and Mn-SOD Assay Kit with WST-8 and Cellular Glutathione Peroxidase Assay Kit with NADPH in METTL7B-overexpressed PC9 (b) and HCC827 cells (c). d The same experiments with (a) but in METTL7B-suppressed PC9-GR, H1975 and PC9-OR cells. e–g The same experiments with (b-c) but shMETTL7B were stably transfected into gefitinib-resistant PC9-GR, H1975 and osimertinib-resistant PC9-OR cells. **P < 0.01, ***P < 0.001 and ****P < 0.0001

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