Fig. 7
METTL7B regulated the stability of ROS-scavenging related genes mediated by m6A modification. a RNA dot blot analysis (upper panel) of m6A levels in METTL7B-overexpressed PC9, HCC827 and METTL7B-suppressed PC9-GR, H1975 and PC9-OR cells. Methylene blue staining served as a loading control (lower panel). b-e m6A immunoprecipitation and qRT-PCR performed to determine the change of GPX4, HMOX1 and SOD1 mRNA with m6A modification. f-l Overexpression of METTL7B in PC9 cells upregulated m6A levels, GPX and SOD activity, with the increased IC50 to gefitinib, and the effect was reversed by 3-DA (10 μM for 72 h). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001