Fig. 4
From: Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification
ALKBH5 regulates PKMYT1 via its m6A-dependent manner (A) Prediction results of PKMYT1 mRNA in SRAMP website show the potential site of m6A modification. The red arrow points sites with very high confidence. (B) MeRIP-qPCR analysis of five sites on PKMYT1 mRNA in HGC-27 cell. GAPDH was regarded as the negative control, while HECBPA belongs to the positive control. (C) Peak distribution of the first two sites in MeRIP profiles of GC tissue in IGV genome browser. (D) Schematic photo of CDS-WT, CDS-mut1, CDS-mut2 in PKMYT1 mRNA. (E–F) The protein and mRNA level of PKMYT1 between NC and shRNA-1 group after transfected with CDS-WT, CDS-mut1 and CDS-mut2 in SGC-7901 cell. (G-H) The migration and invasion ability between NC and shRNA-1 group after transfected with CDS-WT, CDS-mut1 and CDS-mut2 in SGC-7901 cell (scale bar = 200 um)