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Fig. 8 | Molecular Cancer

Fig. 8

From: CDK6-PI3K signaling axis is an efficient target for attenuating ABCB1/P-gp mediated multi-drug resistance (MDR) in cancer cells

Fig. 8

Migration of cdk6 intact and deficient KB-C2 cells to lungs, spleens and livers in the nude mice. A The results of immunofluorescence labeling of CKAP4 showed that the cdk6-deleted cancer cells in the nude mice injected with DOX were the least in number of metastasis spots. DOX had little effect on the metastasis of cancer cells without cdk6 deletion, and had a significant effect on the metastasis of cdk6-deleted cancer cells. All nude mice without DOX injection had serious cancer cell metastasis. B KB-C2-k.o.cdk6 cells were eliminated from the lungs in nude mice after treatment with DOX. The arrows in orange depict the distorted dead KB-C2-k.o.cdk6 cells that had expressed high level of CKAP4. Due to the damage of the nuclei, DAPI fluorescence was absent in these cells. To testify this phenomenon, red and green fluorescence, respective of Cy3 and FITC labeled secondary antibodies, were used to determine antigen CKAP4-abundant KB-C2-k.o.cdk6 cells

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Molecular Cancer

ISSN: 1476-4598