Fig. 4

Circ-ZEB1 binds to miR-199a-3p. A MiR-199a-3p levels within HCC and matched non-carcinoma samples were measured by RT-qPCR. B Circ-ZEB1 expression was positively correlated with miR-199a-3p expression in HCC. C, D Changes in the expression of miR-199a-3p after downregulation of circ-ZEB1 were measured by RT-qPCR. E The FISH assay revealed the co-localization of circ-ZEB1 with miR-199a-3p (^*400). F Circ-ZEB1 enriched with a specific probe detected by RT-qPCR. G The enriched miR-199a-3p RT-qPCR detected circ-ZEB1-specific probe. H Circ-ZEB1 expression was trapped using the biotin-conjugated miR-199a-3p and measured by RT-qPCR. ^*P < 0.05 versus biotin Sh-NC group. All values are presented as mean ± standard deviation (SD). Comparisons between the groups were analyzed by unpaired t-test. This experiment was performed thrice