Fig. 5

Silencing of miR-199a-3p inhibits the regulation of HCC cell proliferation and apoptosis by circ-ZEB1. A The MTT assay was used to detect the activity change in MHCC-97H cells after simultaneous downregulation of circ-ZEB1 and miR-199a-3p. B The MTT assay was used to detect the change in the HCC-LM3 activity after simultaneous downregulation of circ-ZEB1 and miR-199a-3p. C The plate cloning experiment reflects the changes in the proliferation ability of MHCC-97H and HCC-LM3 after simultaneous transfection of cells with Sh-circ-ZEB1 and miR-199a-3p inhibitors. D Changes in the proliferation of MHCC-97H cells after simultaneous transfection of cells with Sh-circ-ZEB1 and miR-199a-3p inhibitors detected by EdU assay. E Changes in the proliferation of HCC-LM3 cells after co-transfection with Sh-circ-ZEB1 and miR-199a-3p inhibitors and detected using EdU assay. F Flow cytometry revealed apoptotic changes in MHCC-97H cells after simultaneous downregulation of circ-ZEB1 and miR-199a-3p. G Flow cytometry revealed apoptotic changes in HCC-LM3 cells after simultaneous downregulation of circ-ZEB1 and miR-199a-3p