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Fig. 6 | Molecular Cancer

Fig. 6

From: Circ-ZEB1 promotes PIK3CA expression by silencing miR-199a-3p and affects the proliferation and apoptosis of hepatocellular carcinoma

Fig. 6

Circ-ZEB1 positively regulates the target gene PIK3CA of miR-199a-3p. A Bioinformatics analysis shows that circ-ZEB1 and PIK3CA have binding sites. B The dual-luciferase reporter assay reveals PIK3CA as the target gene of circ-ZEB1. C Correlation analysis between circ-ZEB1 and PIK3CA. D, E Changes in PIK3CA mRNA expression in circ-ZEB1 knockdown cells detected by RT-qPCR. F Changes in the protein expression of PIK3CA in circ-ZEB1 knockdown cells measured by western blotting. G, H Changes in the mRNA expression of PIK3CA in miR-199a-3p inhibitor-transfected cells measured by RT-qPCR. I Changes in the protein expression of PIK3CA in miR-199a-3p inhibitor-transfected cells measured by western blotting. J The expression change in PIK3CA protein level after simultaneously knocking down circ-ZEB1 and downregulating miR-199a-3p. Statistical analysis was performed by one-way analysis of variance (ANOVA) or repeated-measures ANOVA. Pearson’s correlation analysis was used to correlate the two groups. This experiment was performed thrice

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