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Fig. 1 | Molecular Cancer

Fig. 1

From: PIK3CA mutations-mediated downregulation of circLHFPL2 inhibits colorectal cancer progression via upregulating PTEN

Fig. 1

CircLHFPL2 is downregulated in PIK3CA-mutant CRC cells and tissues. A PIK3CA parental, WT and mutant allele configurations of CRC lines. B The flowchart delineates the steps for identifying and validating circRNAs in PIK3CA-mutant CRC cells. C Hierarchical clustering showing the top 30 upregulated and downregulated circRNAs in HCT116 PIK3CAH1047R (left) and DLD1 PIK3CAE545K (right) cells. D Volcano plots showing differential expression between the two different conditions. E Relative expression of circLHFPL2 determined via qRT-PCR in parental, WT and Mut HCT116 (left) and DLD1 (right) cells. F Relative expression of circLHFPL2 determined via qRT-PCR in CRC cell lines. G Relative expression of circLHFPL2 determined via qRT-PCR in tumor tissues of 30 CRC patients with PIK3CA Mut and 30 patients with PIK3CA WT. H Survival analysis in patients with different expression pattern of circLHFPL2 with Kaplan-Meier method. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01

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Molecular Cancer

ISSN: 1476-4598