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Fig. 2 | Molecular Cancer

Fig. 2

From: PIK3CA mutations-mediated downregulation of circLHFPL2 inhibits colorectal cancer progression via upregulating PTEN

Fig. 2

CircLHFPL2 was the target gene of the transcription factor Foxo3a. A Schematic illustration showing LHFPL2 exon 4 forming circLHFPL2. The existence of circLHFPL2 was proven by RT–PCR and its back-splicing junction was verified by Sanger sequencing. Red arrow indicates the special splicing junction of circLHFPL2. B The existence of circLHFPL2 determined in HCT116 and DLD1 cell lines by qRT-PCR. Divergent primers amplified circLHFPL2 in cDNA but not genomic DNA (gDNA). GAPDH was utilized as an internal control. C The subcellular fraction assay revealed subcellular location of circLHFPL2 in HCT116 and DLD1 cells. D Western blotting showed that SC-79 increased the protein expression level of p-AKT and p-Foxo3a in HCT116 (left) cells and DLD1 (right) cells. E Luciferase reporter assay confirmed the interaction between LHFPL2 promoter and Foxo3a. F Chip assay confirmed the interaction between LHFPL2 and Foxo3a. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01

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Molecular Cancer

ISSN: 1476-4598