Fig. 3

Overexpression of circLHFPL2 inhibited the growth of PIK3CA-mutant CRC cells. A Relative expression of circLHFPL2 determined via qRT-PCR in HCT116 PIK3CA^WT or PIK3CA^H1047R (left) and DLD1 PIK3CA^WT or PIK3CA^E545K cells (right) transfected with circLHFPL2 overexpression lentivirus. B CCK-8 assay revealed the viability of HCT116 PIK3CA^WT or PIK3CA^H1047R (left) and DLD1 PIK3CA^WT or PIK3CA^E545K cells (right) transfected with circLHFPL2 overexpression lentivirus. C Colony formation assay revealed the proliferation of HCT116 PIK3CA^WT or PIK3CA^H1047R (left) and DLD1 PIK3CA^WT or PIK3CA^E545K cells (right) transfected with circLHFPL2 overexpression lentivirus. D Apoptosis assay showed the apoptosis of HCT116 PIK3CA^WT or PIK3CA^H1047R (left) and DLD1 PIK3CA^WT or PIK3CA^E545K cells (right) transfected with circLHFPL2 overexpression lentivirus. E Western blotting showed the protein expression of BCL-2, Bax and cleaved PARP in HCT116 PIK3CA^WT or PIK3CA^H1047R (left) and DLD1 PIK3CA^WT or PIK3CA^E545K cells (right) transfected with circLHFPL2 overexpression lentivirus. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01