Fig. 4

Overexpression of circLHFPL2 inhibited the growth of PIK3CA-mutant CRC xenograft tumor. A Treated cells (3 × 10⁶, 100 μL) were subcutaneously injected into the left flank of the mice. Tumor size was determined as previously reported (Length x Width² × 0.5) every 3 days. After 30 days, the mice were sacrificed, and the tumor weight was measured. B IHC staining showed the protein expression of BCL-2, Bax, cleaved PARP in xenograft tumors. C A mouse model of lung metastases established by tail vein injection of the indicated HCT116 PIK3CA^H1047R and DLDL1 PIK3CA^E545K cells with circLHFPL2 overexpression. Representative bioluminescence images (C, left) and bioluminescence signals (C, right) acquired at 8 weeks after injection. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01