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Fig. 7 | Molecular Cancer

Fig. 7

From: PIK3CA mutations-mediated downregulation of circLHFPL2 inhibits colorectal cancer progression via upregulating PTEN

Fig. 7

Downregulation of circLHFPL2 sustained activation of PI3K/AKT pathway via a positive feedback loop of miR-556-5p and miR-1322/PTEN axis. A CCK-8 assay on the viability of PIK3CAH1047R and PIK3CAE545K cells transfected with circLHFPL2 overexpression plasmid or with the combination of miR-556-5p mimics, miR-1322mimics and siPTEN. B Colony formation assay on the proliferation of PIK3CAH1047R and PIK3CAE545K cells transfected with circLHFPL2 overexpression plasmid or with the combination of miR-556-5p mimics, miR-1322mimics and siPTEN. C Apoptosis assay showed the apoptosis of PIK3CAH1047R and PIK3CAE545K cells transfected with circLHFPL2 overexpression plasmid or with the combination of miR-556-5p mimics, miR-1322mimics and siPTEN. D Western blotting showed the protein expression level of PTEN, BCL-2, Bax and cleaved PARP in PIK3CAH1047R and PIK3CAE545K cells transfected with circLHFPL2 overexpression plasmid or with the combination of miR-556-5p mimics, miR-1322mimics and siPTEN. Data are presented as mean ± SEM; n ≥ 3. *p < 0.05; **p < 0.01

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Molecular Cancer

ISSN: 1476-4598